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Marek Orłowski, PhD
Email: marek.orlowski@pwr.edu.pl
Unit: Faculty of Chemistry » Department of Biochemistry, Molecular Biology and Biotechnology
ul. Gdańska 7/9, 50-344 Wrocław
building F-4, room C-14
phone +48 71 320 6286
Office hours
- Monday 13.15-15.15
- Tuesday 13.15-15.15
Research fields
- Nuclear receptor superfamily; functional ecdysteroid receptor; 20-hydroxyecdysone receptor (EcR); ultraspiracle protein (Usp); signal transduction pathways; transcription factors; intrinsically disordered proteins (IDPs); intrinsically disordered regions (IDRs); analyses of protein - protein and protein - DNA interactions; overexpression of recombinant proteins in heterologous and native systems; purification of recombinant proteins (platform of AKTA brand chromatography systems, IMAC, gel filtration and more); biochemical and structural analyses of proteins using circular dichroism (CD) spectroscopy and bioinformatics tools; analyses of conformational changes in proteins; analyses of subcellular distribution of proteins using eukaryotic cell lines; analyses of molecular determinants of subcellular distribution of proteins: nuclear localization signals (NLSs), nuclear export signals (NESs) and nucleolus localization signals (NoLSs); fluorescence and confocal microscopy.
Recent papers
2018
- Orłowski M., Popławska K., Pieprzyk J., Szczygieł-Sommer A., Więch A., Zarębski M., Tarczewska A., Dobrucki J. & Ożyhar A. 2018, Molecular determinants of Drosophila immunophilin FKBP39 nuclear localization, Biological Chemistry, DOI: https://doi.org/10.1515/hsz-2017-0251.
2017
- Kozłowska M., Tarczewska A., Jakób M., Bystranowska D., Taube M., Kozak M., Czarnocki-Cieciura M., Dziembowski A., Orłowski M., Tkocz K. & Ożyhar, A. 2017, Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments, Scientific Reports, vol. 7, pp. 40405.
2014
- Pieprzyk J., Zbela A., Jakób M., Ożyhar A. & Orłowski M. 2014, Homodimerization propensity of the intrinsically disordered N-terminal domain of Ultraspiracle from Aedes aegypti, Biochimica et Biophysica Acta, vol. 1844, no. 6, pp. 1153-1166.
2007
- Jakób M., Kołodziejczyk R., Orłowski M., Krzywda S., Kowalska A., Dutko-Gwóźdź J., Gwóźdź T., Kochman M., Jaskólski M. & Ożyhar A. 2007, Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain, Nucleic Acids Research, vol. 35, no. 8, pp. 2705-2718.
2004
- Orłowski M., Szyszka M., Kowalska A., Grad I., Zoglowek A., Rymarczyk G., Dobryszycki P., Krowarsch D., Rastinejad F., Kochman M. & Ożyhar A. 2004, Plasticity of the ecdysone receptor DNA binding domain, Molecular Endocrinology, vol. 18, no. 9, pp. 2166-2184.